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Updated: May 16, 2026
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Dynamic quantitative phase imaging for biological objects using a pixelated phase mask
Katherine Creath1, Goldie Goldstein
14D Technology Corp, 3280 E Hemisphere Loop, Ste 146, Tucson, AZ 85706, USA ; College of Optical Sciences, The University of Arizona, 1680 E University Blvd, Tucson, AZ 85721, USA ; Optineering, 2247 E La Mirada St, Tucson, AZ 85719, USA.
Abstract:
This paper describes research in developing a dynamic quantitative phase imaging microscope providing instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns derived using the polarization properties of light to track dynamic motions and morphological changes. Optical path difference (OPD) and optical thickness (OT) data are obtained from phase images. Two different processing routines are presented to remove background surface shape to enable quantification of changes in cell position and volume over time. Data from a number of different moving biological organisms and cell cultures are presented.
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