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Published on: March 11, 2020
Overview of tomato (Solanum lycopersicum) candidate pathogen recognition genes reveals important Solanum R locus
G Andolfo1, W Sanseverino1, S Rombauts2
1Department of Soil, Plant, Environmental and Animal Production Sciences, University of Naples 'Federico II', Via Universita 100, 80055, Portici, Italy.
The New Phytologist
|November 21, 2012
Summary
This study catalogs plant immune receptor genes in tomato and potato, revealing how gene clusters evolve to enhance pathogen defense. Differences in gene arrangement between species highlight ongoing adaptation in Solanum R loci.
Area of Science:
- Plant genetics
- Molecular evolution
- Genomics
Background:
- Plant immune receptors, including nucleotide-binding site (NBS), receptor-like protein (RLP), and receptor-like kinase (RLK) genes, are crucial for recognizing pathogens.
- Understanding the genome-wide organization and evolution of these R loci is key to deciphering plant defense mechanisms.
Purpose of the Study:
- To generate a comprehensive catalog of NBS, RLP, and RLK genes in tomato (Solanum lycopersicum) and potato (Solanum tuberosum).
- To investigate the structural diversity, phylogenetic relationships, and chromosomal distribution of these candidate pathogen recognition genes.
- To explore the evolutionary mechanisms shaping the architecture of R loci and their role in plant defense.
Main Methods:
- Genome-wide cataloging of NBS, RLP, and RLK gene repertoires in tomato and potato.
- Characterization of candidate pathogen recognition genes, including structural diversity, phylogenetic analysis, and chromosomal mapping.
- Comparative analysis of R loci organization and evolution between tomato and potato.
Main Results:
- NBS genes often form clusters with RLP or RLK genes, suggesting selection for coordinated transcription.
- Duplication events and lineage-specific evolution were identified, contributing to the expansion and modulation of the plant defense repertoire.
- Significant differences in the number and organization of candidate pathogen recognition genes were observed between tomato and potato, with evidence of ongoing lineage-specific rearrangement and a local expansion of Toll/Interleukin-1 receptor (TIR)-NBS-leucine-rich repeat (LRR) (TNL) genes in potato.
Conclusions:
- Evolutionary mechanisms, including gene duplication and rearrangement, have shaped the architecture of pathogen recognition gene clusters in Solanum species.
- Comparative analysis reveals distinct evolutionary trajectories and adaptive divergence in R loci between tomato and potato.
- These findings provide insights into molecular adaptive selection at Solanum R loci, crucial for understanding plant immunity.
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