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Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
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Recent progress in biocatalysis using supercritical carbon dioxide.

Tomoko Matsuda1

  • 1Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Japan. tmatsuda@bio.titech.ac.jp

Journal of Bioscience and Bioengineering
|November 21, 2012
PubMed
Summary

This review covers advances in biocatalysis using supercritical carbon dioxide (scCO(2)). It details enzyme stability and organic synthesis reactions, focusing on lipase-catalyzed chiral compound synthesis and other enzymatic processes in scCO(2).

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Area of Science:

  • Biocatalysis
  • Green Chemistry
  • Enzyme Engineering

Background:

  • Supercritical carbon dioxide (scCO(2)) offers unique properties as a reaction medium for enzymatic processes.
  • Understanding enzyme stability and developing stabilization strategies are crucial for effective biocatalysis in scCO(2).
  • scCO(2) presents opportunities for novel organic synthesis pathways.

Purpose of the Study:

  • To review recent advancements in biocatalysis utilizing supercritical carbon dioxide.
  • To discuss enzyme stability and stabilization techniques in scCO(2) environments.
  • To highlight various organic synthesis reactions catalyzed by enzymes in scCO(2).

Main Methods:

  • Literature review of biocatalysis research employing supercritical carbon dioxide.
  • Analysis of enzyme stability and immobilization methods in scCO(2).
  • Compilation of examples of enzymatic organic synthesis reactions performed in scCO(2).

Main Results:

  • Enzymes exhibit varying stability in scCO(2), with specific stabilization methods enhancing their performance.
  • Lipase-catalyzed synthesis of chiral compounds is a prominent application, with numerous examples provided.
  • Other enzymatic reactions, including asymmetric reduction by alcohol dehydrogenase and carboxylation by decarboxylase, are feasible in scCO(2).

Conclusions:

  • Supercritical carbon dioxide is a versatile medium for advanced biocatalysis.
  • Enzyme stabilization is key to unlocking the full potential of biocatalysis in scCO(2).
  • The reviewed enzymatic reactions demonstrate the utility of scCO(2) in green organic synthesis.