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Related Experiment Videos

Five polymorphic microsatellite VNTRs on the human X chromosome.

J A Luty1, Z Guo, H F Willard

  • 1Department of Biochemistry, Oregon Health Sciences University, Portland 97201-3098.

American Journal of Human Genetics
|April 1, 1990
PubMed
Summary

Researchers identified numerous microsatellite variable number of tandem repeats (VNTRs) on the X chromosome. These genetic markers are highly polymorphic and useful for genetic studies, unlike classical VNTRs.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Human Genome Research

Background:

  • The human genome contains abundant interspersed repeats, including microsatellite variable number of tandem repeats (VNTRs).
  • Microsatellite VNTRs exhibit length polymorphism among individuals and can be analyzed using PCR.
  • Few VNTRs have been identified on the X chromosome, limiting genetic studies.

Purpose of the Study:

  • To screen for and characterize microsatellite VNTRs on the human X chromosome.
  • To assess the polymorphic potential of X-linked microsatellites for genetic mapping.

Main Methods:

  • Screening of an X chromosome-specific genomic library using a (dT-dG).poly(dA-dC) probe.
  • PCR amplification and DNA sequencing gel analysis of candidate microsatellites.

Related Experiment Videos

  • Localization and regional mapping of VNTRs using PCR on hybrid cell panels and linkage analysis.

    • Main Results:

    • Approximately 25% of screened phage clones contained microsatellite repeats.
    • Five out of seven identified X-linked microsatellites were polymorphic.
    • Three microsatellites displayed high heterozygosity (>75%) and numerous alleles (≥8).
    • Four VNTRs were regionally mapped to specific X chromosome locations; a fifth was mapped via linkage.

    Conclusions:

    • Microsatellite VNTRs are abundant and polymorphic on the X chromosome.
    • The factors limiting classical VNTRs on the X chromosome do not affect microsatellite VNTRs.
    • These findings provide valuable genetic markers for X chromosome studies and mapping.