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Updated: May 16, 2026

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
Published on: March 16, 2012
Development of super-dense transfected cell microarrays generated by piezoelectric inkjet printing
Satoshi Fujita1, Reiko Onuki-Nagasaki, Junji Fukuda
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. s-fujita@aist.go.jp
Abstract:
Super-dense transfected cell microarrays (TCMs) were created by a piezoelectric inkjet printer on a glass substrate that had been grafted with poly(ethylene glycol) (PEG). The micro-spots that contained plasmid and extra-cellular matrix (ECM) protein were separated from one another by a hydrophilic barrier generated by PEG. We successfully constructed the densest TCMs with spots of 50 μm in diameter and 150 μm in pitch.

