Related Experiment Video
Updated: May 16, 2026

Aerobic Biodegradation Testing of Materials Using a Natural Marine Seawater Inoculum and Closed Loop Respirometer
Published on: October 24, 2025
Removal and relationships of microbial indicators in a water treatment and reclamation facility
Laura Alcalde1, Montserrat Folch, Josefina C Tapias
1Soil Science Laboratory, University of Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain. lalcalde@ub.edu
Abstract:
A wastewater treatment and reclamation facility in north-east Spain was monitored over 1 year to determine the occurrence and concentrations of different microbial indicators (Escherichia coli, fecal enterococci, somatic bacteriophages and spores of sulfite-reducing clostridia). The removal of the indicators and its relationships through the wastewater treatment and reclamation trains were evaluated. The results obtained show that the reclamation treatments evaluated present a different efficiency in indicator microorganisms' removal depending on the type of microorganism. The E. coli and enterococci present an average reduction slightly higher than the other indicators, followed by somatic bacteriophages and spores of sulfite-reducing clostridia. The Spearman's correlations indicate that it is not suitable to use any of the bacterial indicators evaluated to predict the content of virus or spores of sulfite-reducing clostridia. Therefore, in order to evaluate the microbiological risk of the reclaimed effluent use, it is necessary to monitor the three types of indicator microorganisms (bacteria, virus and protozoa).
Related Concept Videos
Environmental Applications of Microorganisms
Microbial Bioremediation of Uranium
Bioremediation
Microbial Bioremediation of Pesticides
Microbial Growth Measurement: Indirect Methods
Microbial Bioremediation of Hydrocarbons

