Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial Transcriptomics Recontextualizes the Cellular Environment of Conjunctival Melanoma.

medRxiv : the preprint server for health sciences·2026
Same author

Feasibility of co-polarizing ¹³C-pyruvate and ¹³C-tert-butanol for simultaneous metabolic and perfusion imaging.

Npj imaging·2026
Same author

Hemiplasy helps explain high rates of apparent morphological convergence in neoavian birds.

Current biology : CB·2026
Same author

Across-cities transportable <sup>13</sup>C hyperpolarization using UV-induced labile radicals.

Nature communications·2026
Same author

Suppressing loop current of shielded loops at fundamental resonance.

Scientific reports·2026
Same author

Mandibular morphology clarifies phylogenetic relationships near the origin of crown birds.

BMC ecology and evolution·2025
Same journal

Editorial for "A Quantitative Modification of VI-RADS for Bladder Cancer at the Ureteral Orifice: A Reader Study on MRI With Varying Experience Levels".

Journal of magnetic resonance imaging : JMRI·2026
Same journal

RF Noise Artifacts From a High Frequency Spinal Cord Stimulation System: A Retrospective Imaging Cohort With Illustrative Cases.

Journal of magnetic resonance imaging : JMRI·2026
Same journal

Editorial for "Association Between HIV Infection Duration and Left Ventricular Concentric Remodeling and Myocardial Fibrosis: A Cross-Sectional Study Using Cardiac MRI".

Journal of magnetic resonance imaging : JMRI·2026
Same journal

Editorial for "Effect of Visualized Respiratory Training on Patient Cooperation and Image Quality in Gadoxetic Acid Disodium-Enhanced Liver MRI: A Randomized Controlled Trial".

Journal of magnetic resonance imaging : JMRI·2026
Same journal

Editorial for "Association of Iron and Myelin Alterations in the Contralesional Dentate Nucleus and Thalamus With Functional Outcome in Acute Ischemic Stroke: A Susceptibility Source Separation Study".

Journal of magnetic resonance imaging : JMRI·2026
Same journal

Editorial for "Association Between Deep Gray Matter Iron Deposition Dysregulation and Cognitive Performance in Type 2 Diabetes Mellitus Patients: A Quantitative Susceptibility Mapping Study".

Journal of magnetic resonance imaging : JMRI·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Hyperpolarized 13C metabolic imaging using dissolution dynamic nuclear polarization.

Ralph E Hurd1, Yi-Fen Yen, Albert Chen

  • 1GE Healthcare, Menlo Park, California, USA. ralph.hurd@ge.com

Journal of Magnetic Resonance Imaging : JMRI
|November 21, 2012
PubMed
Summary
This summary is machine-generated.

Dissolution dynamic nuclear polarization (DNP) significantly enhances magnetic resonance imaging (MRI) sensitivity, enabling real-time metabolic studies. This hyperpolarization technique offers a 10,000-100,000x signal boost for clinical applications.

More Related Videos

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Related Experiment Videos

Last Updated: May 16, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Area of Science:

  • Physics
  • Medical Imaging
  • Biochemistry

Background:

  • Magnetic Resonance Imaging (MRI) suffers from low sensitivity.
  • Dynamic Nuclear Polarization (DNP) is a technique to enhance nuclear spin polarization.
  • Dissolution DNP (dDNP) allows for the transfer of hyperpolarized agents for in vivo imaging.

Purpose of the Study:

  • To explain the physics of dissolution DNP.
  • To detail the impact of hyperpolarized states on MRI detection.
  • To review hardware requirements for clinical translation and applications of hyperpolarized agents.

Main Methods:

  • Description of the fundamental physics of dissolution DNP.
  • Explanation of how hyperpolarization impacts MRI signal detection.
  • Review of hardware considerations for clinical implementation.

Main Results:

  • Hyperpolarization provides a 10,000-100,000 fold signal-to-noise ratio (SNR) advantage in MRI.
  • This enhancement overcomes intrinsic sensitivity limitations for a T(1)-dependent observation window.
  • Enables real-time measurements of perfusion, metabolite transport, exchange, and metabolism.

Conclusions:

  • Dissolution DNP significantly boosts MRI sensitivity, enabling new diagnostic capabilities.
  • Hyperpolarized agents allow for real-time metabolic imaging.
  • Applications are progressing in oncology, cardiology, and neurology.