Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

Spermatogenesis

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...

Meiosis II

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Perforated ascending colonic mucinous adenocarcinoma with a subserosal mucin pool presenting as a giant cystic lesion: a case report.

International journal of surgery case reports·2026

Same author

Risk Factors and Preventive Measures for Well-Leg Compartment Syndrome During Minimally Invasive Surgery in the Lithotomy Position.

Journal of clinical medicine·2026

Same author

Parthenogenote-derived brain unveils the critical role of paternal genome in neural development.

Scientific reports·2026

Same author

Efficacy of Triclosan-Coated Barbed Sutures for Fascial Closure in Colorectal Surgery.

The Journal of surgical research·2026

Same author

A Case of Pleomorphic-Type Anaplastic Carcinoma of the Pancreas with Rapidly Progressive and Fatal Cardiac Metastasis.

Surgical case reports·2026

Same author

Impact of an individualized camera port position adjustment on short-term outcomes in robotic gastrectomy.

Surgery today·2025

Search research articles

Related Experiment Video

Updated: May 16, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

piRNA and spermatogenesis in mice.

Shinichiro Chuma¹, Toru Nakano

¹Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences

|November 21, 2012

Summary

This summary is machine-generated.

The piwi-small RNA pathway in mice safeguards the germline genome from retrotransposons. This pathway is crucial for spermatogenesis, male fertility, and maintaining genome integrity.

More Related Videos

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Published on: September 17, 2019

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

Related Experiment Videos

Last Updated: May 16, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Published on: September 17, 2019

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

Area of Science:

Genetics
Molecular Biology
Reproductive Biology

Background:

Transposable elements constitute a significant portion of mammalian genomes, posing a genetic threat.
Host genomes employ defense mechanisms like epigenetic regulation and RNA silencing to control mobile elements.
The piwi-small RNA pathway is vital for germline genome protection against retrotransposons.

Purpose of the Study:

To review the current understanding of the piwi pathway in mice.
To highlight the role of the piwi pathway in spermatogenesis and male fertility.
To discuss the link between small RNAs and transcriptional control in mammals.

Main Methods:

Review of recent studies on the piwi pathway in mice.
Analysis of the role of vasa and tudor family genes in piwi pathway function.
Examination of epigenetic effects of the piwi pathway on genome loci.

Main Results:

The piwi pathway is essential for suppressing retrotransposons in germ cells.
Developmentally regulated genes like vasa and tudor are key players in the piwi pathway.
The piwi pathway influences both RNA levels and epigenetic modifications of target genes.

Conclusions:

The piwi pathway is a fundamental component of spermatogenesis, ensuring male fertility and genome integrity.
Understanding the piwi pathway provides insights into germline development and genome defense.
The piwi pathway links small RNA activity to transcriptional control in mammals.