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Related Experiment Videos

Pseudoketogenesis in hepatectomized dogs.

C Des Rosiers1, J A Montgomery, M Garneau

  • 1Department of Nutrition, University of Montreal, Quebec, Canada.

The American Journal of Physiology
|March 1, 1990
PubMed
Summary

Pseudoketogenesis, or label exchange in extrahepatic tissues, can overestimate ketone body turnover. This study demonstrates the reversibility of 3-oxoacid-CoA transferase, supporting the concept of pseudoketogenesis in vivo.

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Area of Science:

  • Biochemistry
  • Metabolic Research
  • Animal Models

Background:

  • Ketone body turnover is crucial for energy metabolism.
  • Tracer kinetics are commonly used to measure in vivo turnover.
  • Potential for overestimation exists due to metabolic pathway complexities.

Purpose of the Study:

  • To investigate the phenomenon of pseudoketogenesis.
  • To determine if label exchange via 3-oxoacid-CoA transferase causes overestimation of ketone body turnover.
  • To quantify the extent of this dilution in extrahepatic tissues.

Main Methods:

  • Euglycemic hepatectomized dogs were used as a model.
  • A bolus of [3,4-13C2]acetoacetate was administered.
  • Blood acetoacetate and R-3-hydroxybutyrate labeling patterns were analyzed using gas chromatography-mass spectrometry.

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Main Results:

  • Molar percent enrichment of [3,4-13C2]acetoacetate significantly decreased in controls and dichloroacetate-treated dogs.
  • Enrichment of R-3-hydroxy-[3,4-13C2]butyrate mirrored that of acetoacetate.
  • Uniformly labeled acetoacetate indicated equilibration between acetoacetate and acetyl-CoA, confirming 3-oxoacid-CoA transferase reversibility.

Conclusions:

  • The reversibility of 3-oxoacid-CoA transferase in extrahepatic tissues was demonstrated.
  • The concept of pseudoketogenesis, leading to overestimated ketone body turnover, is supported.
  • Kinetic analysis quantifies this metabolic phenomenon.