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Characterization of protein higher order structure using vibrational circular dichroism spectroscopy

Radhika P Nagarkar1, Brian M Murphy, Xiaotong Yu

  • 1Bioanalytical Sciences, Biopharmaceutical Development R & D, GlaxoSmithKline, 709 Swedeland Rd, King of Prussia, PA 19406, USA.

Current Pharmaceutical Biotechnology
|November 22, 2012
PubMed
Summary

Vibrational circular dichroism (VCD) and Fourier Transform Infrared (FTIR) spectroscopy monitor protein higher order structures (HOS). VCD effectively detects subtle secondary structure changes in therapeutic proteins during processing.

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