Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improve genetic quality control to increase rigor and reproducibility of mouse research.

Science (New York, N.Y.)·2026
Same author

Modeling patient variants of <i>Cnot1</i> and <i>Cdc42bpb</i> results in distinct forms of congenital diaphragmatic hernia in mice.

bioRxiv : the preprint server for biology·2026
Same author

Data-driven prioritization of mouse strains for improved preclinical modeling of rare and common disease.

bioRxiv : the preprint server for biology·2026
Same author

Evolutionary transfer learning enables organism-wide inference of mammalian enhancer landscapes.

bioRxiv : the preprint server for biology·2026
Same author

Towards clinical translation of nanomedicines: Formulation scale-up and model systems.

Advanced drug delivery reviews·2026
Same author

Efficient <i>in vivo</i> mammalian neuron editing using peptide-mediated CRISPR enzyme delivery.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: May 16, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource.

Caleb S Heffner1, C Herbert Pratt, Randal P Babiuk

  • 1The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.

Nature Communications
|November 22, 2012
PubMed
Summary
This summary is machine-generated.

Characterizing Cre-driver lines is crucial for utilizing loxP-flanked alleles. A new high-throughput pipeline reveals widespread, often unreported, Cre recombinase activity in many mouse strains, impacting genetic research.

More Related Videos

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
10:29

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Published on: November 17, 2017

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

Related Experiment Videos

Last Updated: May 16, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
10:29

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Published on: November 17, 2017

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • The International Knockout Mouse Consortium generates valuable loxP-flanked alleles.
  • Effective use of these alleles depends on well-characterized Cre-driver lines.
  • Existing Cre-driver lines often exhibit off-target or uncharacterized Cre recombinase activity.

Purpose of the Study:

  • To develop and implement a high-throughput pipeline for comprehensive characterization of Cre-driver lines.
  • To document Cre recombinase activity across a wide range of tissues and time points.
  • To disseminate this critical information to the scientific community.

Main Methods:

  • Establishment of a high-throughput pipeline for Cre-driver line analysis.
  • Systematic assessment of Cre recombinase excision activity in diverse tissues.
  • Documentation of activity at multiple time points post-induction.

Main Results:

  • The majority of tested Cre-driver strains showed some level of uncharacterized Cre activity.
  • Mosaicism, inconsistent recombination, and parent-of-origin effects were frequently observed.
  • Significant off-target recombination was detected in multiple tissues.

Conclusions:

  • Deep characterization of Cre-driver lines is essential for accurate genetic manipulation.
  • The developed pipeline provides a critical resource for assessing Cre-driver line reliability.
  • This work underscores the need for thorough validation to ensure the integrity of knockout mouse models.