Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Muscle-Derived Extracellular Vesicles Influence Motor Neuron Regeneration Accuracy.

Neuroscience·2019
Same author

The title and data of Robinson and Madison (2016) are valid.

Journal of neuroscience research·2016
Same author

Polyethylene glycol fusion repair prevents reinnervation accuracy in rat peripheral nerve.

Journal of neuroscience research·2016
Same author

Adolescent Intermittent Alcohol Exposure: Dysregulation of Thrombospondins and Synapse Formation are Associated with Decreased Neuronal Density in the Adult Hippocampus.

Alcoholism, clinical and experimental research·2015
Same author

Extracellular vesicles from a muscle cell line (C2C12) enhance cell survival and neurite outgrowth of a motor neuron cell line (NSC-34).

Journal of extracellular vesicles·2014
Same author

In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.

Journal of neuroscience methods·2014

Related Experiment Video

Updated: May 16, 2026

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Polysialic acid expression is not necessary for motor neuron target selectivity.

Grant A Robinson1, Roger D Madison

  • 1Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA.

Muscle & Nerve
|November 22, 2012
PubMed
Summary

Axon regeneration after nerve injury can be guided to targets. This study shows that motor neurons can selectively target muscles even without upregulating polysialic acid, challenging previous theories.

More Related Videos

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons
10:57

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons

Published on: July 13, 2015

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes
13:36

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

Published on: October 20, 2023

Related Experiment Videos

Last Updated: May 16, 2026

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons
10:57

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons

Published on: July 13, 2015

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes
13:36

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

Published on: October 20, 2023

Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Peripheral nerve injury recovery relies on guiding regenerating axons to their correct targets.
  • Upregulation of polysialic acid by regrowing axons has been hypothesized as crucial for target selectivity.

Purpose of the Study:

  • To investigate the role of polysialic acid in target selectivity during peripheral nerve regeneration.
  • To determine if motor neurons that do not upregulate polysialic acid can achieve selective reinnervation.

Main Methods:

  • Utilized a cross-reinnervation model in rodents.
  • Obturator motor neuron axons, which do not typically upregulate polysialic acid, were directed to regenerate into the distal femoral nerve.
  • Assessed target selectivity between muscle and skin pathways.

Main Results:

  • Without pathway manipulation, obturator motor neurons showed no preference between muscle and skin pathways.
  • When the skin pathway was shortened, these motor neurons selectively targeted muscle.
  • This selective muscle targeting occurred independently of polysialic acid upregulation.

Conclusions:

  • Intrinsic molecular properties of motor neuron pools can be overridden by altering their peripheral nerve pathway access.
  • Obturator motor neurons can preferentially innervate muscle targets even without expressing polysialic acid.
  • The study demonstrates a polysialic acid-independent mechanism for achieving target selectivity in nerve regeneration.