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Related Concept Videos

Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

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Related Experiment Video

Updated: May 16, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

The β-catenin destruction complex.

Jennifer L Stamos1, William I Weis

  • 1Departments of Structural Biology and Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Cold Spring Harbor Perspectives in Biology
|November 22, 2012
PubMed
Summary

The Wnt/β-catenin pathway relies on a destruction complex for regulation. This review explores the complex

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Cancer Research

Background:

  • The Wnt/β-catenin pathway is crucial for cellular processes and is tightly regulated.
  • Aberrant Wnt/β-catenin signaling is implicated in various cancers.
  • A multiprotein 'destruction complex' normally degrades β-catenin in the absence of Wnt stimulus.

Purpose of the Study:

  • To review the molecular mechanisms governing the Wnt/β-catenin destruction complex.
  • To elucidate the specific roles of Adenomatous Polyposis Coli (APC) within this complex.
  • To highlight areas of the destruction complex's function that require further investigation.

Main Methods:

  • Literature review of molecular mechanisms.
  • Analysis of protein interactions within the destruction complex.

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An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
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An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

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Last Updated: May 16, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

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  • Discussion of the functional significance of key components, particularly APC.

    • Main Results:

    • The destruction complex, including Axin, APC, GSK-3, CK1, PP2A, and β-TrCP, facilitates β-catenin degradation.
    • Phosphorylation of β-catenin by kinases creates a recognition site for β-TrCP, leading to proteasomal degradation.
    • The precise role of APC in β-catenin destruction remains incompletely understood.

    Conclusions:

    • The Wnt/β-catenin pathway's regulation is essential for proper gene activation.
    • Further research is needed to fully understand the molecular mechanisms of the destruction complex, especially the function of APC.
    • Clarifying APC's role could offer new therapeutic targets for Wnt pathway-driven diseases.