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Updated: May 16, 2026
12:09
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Published on: August 10, 2022
Concise review: Bone marrow meets blastocyst: lessons from an unlikely encounter
Bert Binas1, Catherine M Verfaillie
1Division of Molecular and Life Science, Hanyang University, Kyeonggi-do, South Korea. bbinas@hanyang.ac.kr
Stem Cells (Dayton, Ohio)
|November 22, 2012
Summary
Rat multipotent adult progenitor cells (rMAPCs) are similar to extraembryonic endoderm precursor cells (rXENP). This discovery clarifies rMAPC lineage identity and highlights stem cell plasticity, crucial for regenerative medicine.
Area of Science:
- Stem cell biology
- Developmental biology
- Regenerative medicine
Background:
- Rat multipotent adult progenitor cells (rMAPCs) have broad differentiation potential but uncertain lineage.
- Rat blastocyst-derived extraembryonic endoderm precursor (rXENP) cells represent committed extraembryonic endoderm precursors.
Purpose of the Study:
- To investigate the relationship between rMAPCs and rXENP cells.
- To assign a lineage identity to rMAPCs and explore stem cell plasticity.
Main Methods:
- Homogeneous culturing of rXENP cells under rMAPC conditions.
- Derivation of rat hypoblast stem cells (rHypoSCs) from rat blastocysts.
- Comparative analysis of gene expression profiles and developmental potentials of rHypoSCs, rXENP cells, and rMAPCs.
Main Results:
- rXENP cells can be cultured homogeneously under rMAPC conditions.
- Rat hypoblast stem cells (rHypoSCs) were derived more rapidly and directly from blastocysts.
- rHypoSCs, rXENP cells, and rMAPCs exhibit highly similar gene expression profiles and developmental potentials.
- Lineage identity was assigned to rMAPCs, suggesting origin by environmental reprogramming.
Conclusions:
- rMAPCs, rXENP cells, and rHypoSCs possess lineage plasticity.
- The findings strengthen the consistency between rat and mouse embryology regarding hypoblast precursors.
- Pure hypoblast stem cells offer a model for studying pluripotency, plasticity, and transplantation tolerance in regenerative medicine.
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