Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Titration in Nonaqueous Solvents01:16

Titration in Nonaqueous Solvents

Most acid-base titrations are performed in an aqueous medium. In aqueous titrations, water competes with weaker acids or bases for proton donation or acceptance, leading to ambiguous endpoints in the titration curve. Water also affects the partial ionization of weak acids or bases. For example, water accepts a proton from acetic acid to form hydronium and acetate ions. The hydronium ion formed is a stronger acid than acetic acid, and the acetate ion is a stronger base than water. As a result,...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Update of descriptors for organosilicon compounds compatible with the 2025-WSU compound descriptor database for use with the solvation parameter model.

Journal of chromatography. A·2026
Same author

Addition and update of compounds for the 2025 Wayne state university compound descriptor database for use with the solvation parameter model.

Journal of chromatography. A·2026
Same author

Update and expansion of a system constant database for biphasic liquid-liquid partition systems.

Journal of chromatography. A·2025
Same author

Update and expansion of a system constant database for open-tubular columns used in gas chromatography.

Journal of chromatography. A·2025
Same author

Update of the Wayne State University system constant database for reversed-phase liquid chromatography columns for varied mobile phase compositions.

Journal of chromatography. A·2025
Same author

A comparison of the Abraham and Wayne state university compound descriptor databases for use with the solvation parameter model.

Journal of chromatography. A·2025

Related Experiment Video

Updated: May 16, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Totally organic biphasic solvent systems for extraction and descriptor determinations.

Colin F Poole1, Thushara Karunasekara, Thiloka C Ariyasena

  • 1Department of Chemistry, Wayne State University, Detroit, MI, USA. cfp@chem.wayne.edu

Journal of Separation Science
|November 22, 2012
PubMed
Summary

Totally organic biphasic systems expand liquid-liquid partition applications for challenging compounds. This review covers their characterization and use in sample prep and descriptor determination.

More Related Videos

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

Related Experiment Videos

Last Updated: May 16, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Liquid-liquid partition is crucial for sample prep and analysis.
  • Traditional methods often use water, limiting applications for hydrophobic or unstable compounds.
  • Totally organic biphasic systems offer an expanded scope.

Purpose of the Study:

  • To review totally organic biphasic systems for liquid-liquid partition.
  • To discuss their characterization using the solvation parameter model.
  • To highlight applications in sample preparation and descriptor determination.

Main Methods:

  • Characterization of totally organic biphasic systems.
  • Application of the solvation parameter model.
  • Review of existing literature on system applications.

Main Results:

  • Totally organic biphasic systems broaden the applicability of liquid-liquid partition.
  • These systems are effective for compounds with low water solubility or stability.
  • The solvation parameter model provides a framework for system characterization.

Conclusions:

  • Totally organic biphasic systems represent a significant advancement in liquid-liquid partition.
  • They enhance capabilities in sample preparation and analytical method development.
  • Further research can optimize these systems for diverse chemical analyses.