Simultaneous measurement of SUMOylation using SNAP/CLIP-tag-mediated translation at the single-molecule level

Two opposing SUMO wrestlers: simultaneous measurement of multiple SUMOylations was achieved at the single-molecule level by integrating SNAP/CLIP-tag-mediated translation with single-molecule detection (SUMO=small ubiquitin-like modifier). This method gives exceptional sensitivity, and is capable of measuring the SUMOylation of different proteins under various physiological conditions.