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Related Concept Videos

Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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Second harmonic generation reveals matrix alterations during breast tumor progression.

Kathleen Burke1, Ping Tang, Edward Brown

  • 1University of Rochester, Department of Biomedical Engineering, Rochester, NY 14627, USA.

Journal of Biomedical Optics
|November 23, 2012
PubMed
Summary

Breast cancer stromal changes, measured by collagen

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Area of Science:

  • Biomedical Engineering
  • Cancer Biology
  • Materials Science

Background:

  • Extracellular matrix (ECM) alterations in tumor stroma impact cancer cell metastasis.
  • Understanding breast tumor stromal development is crucial for improving cancer treatment.
  • Collagen, a key ECM component, plays a significant role in tumor progression.

Purpose of the Study:

  • To investigate collagen changes in breast tumor stroma during cancer development.
  • To utilize second harmonic generation (SHG) imaging for quantitative analysis of collagen.
  • To correlate collagen alterations with different types and stages of breast cancer.

Main Methods:

  • Second Harmonic Generation (SHG) imaging of collagen.
  • Quantitative analysis of the forward to backward propagating SHG signal ratio (F/B ratio).
  • Monitoring collagen changes throughout ductal and lobular carcinoma progression.

Main Results:

  • A significant decrease in the collagen F/B ratio was observed in invasive ductal carcinoma, but not in situ ductal carcinoma, compared to healthy tissue.
  • The collagen F/B ratio evolved significantly during the progression of both ductal and lobular carcinomas.
  • Distinct patterns of collagen alteration were noted between ductal and lobular carcinoma progression.

Conclusions:

  • The study provides quantitative insights into stromal reorganization during breast tumor development.
  • Collagen F/B ratio serves as a sensitive indicator of matrix changes in breast cancer.
  • Differences in collagen manipulation between carcinoma types may influence tissue penetration and metastatic potential.