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2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain
Matias Pasquali1, Tommaso Serchi, Jenny Renaut
1Centre de Recherche Public-Gabriel Lippmann, Belvaux, Luxembourg.
Abstract:
Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.
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