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Related Experiment Video

Updated: May 16, 2026

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Published on: May 1, 2019

SAGA complex components and acetate repression in Aspergillus nidulans.

Paraskevi Georgakopoulos¹, Robin A Lockington, Joan M Kelly

¹School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5006, Australia.

G3 (Bethesda, Md.)

|November 23, 2012

Summary

Acetate causes repression in Aspergillus nidulans, distinct from glucose repression. New mutations reveal acetate repression involves CreA and metabolic changes, not independent regulation.

Keywords:

SAGA complex acetate repression carbon catabolite repression creA creB creC

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Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

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Published on: May 1, 2019

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Published on: July 24, 2021

Area of Science:

Molecular Biology
Mycology
Gene Regulation

Background:

Carbon catabolite repression is a well-known regulatory mechanism in fungi.
Acetate, besides sugars, also induces repression in Aspergillus nidulans.
The roles of creA, creB, and creC genes in acetate repression are partially understood.

Purpose of the Study:

To identify novel genes involved in acetate repression in Aspergillus nidulans.
To elucidate the regulatory mechanism of acetate repression.
To differentiate acetate repression from glucose repression pathways.

Main Methods:

Mutational screening using acetate instead of glucose to identify affected genes.
Growth tests and phenotypic analysis of mutant strains (acdX, sptC, facA).
Reverse-transcription quantitative real-time PCR (RT-qPCR) to analyze gene expression (alcA, aldA).

Main Results:

Mutations in acdX and sptC homologs of yeast SAGA components showed derepression for acetate but not glucose.
Acetate metabolism is required for repression of proline metabolism but not acetamide metabolism.
RT-qPCR revealed elevated induced levels, not derepression, of alcohol dehydrogenase genes (alcA, aldA) in acdX and sptC mutants.

Conclusions:

Acetate repression in Aspergillus nidulans is a complex process.
It involves the transcriptional repressor CreA and metabolic alterations.
Acetate repression is not mediated by an independent regulatory control mechanism.