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A two-dimensional acrylamide gel electrophoresis/computer software approach to decoding the human genome
M R Krauss1, P J Collins, S H Blose
1Protein Databases, Inc., Huntington Station, NY 11746.
Biotechniques
|February 1, 1990
Summary
This study proposes using two-dimensional gel electrophoresis and computer software to identify gene protein products. This method aids in efficiently sequencing crucial genes for the human genome project.
Area of Science:
- Genomics
- Proteomics
- Biochemistry
Background:
- The human genome sequencing initiative is a significant undertaking requiring efficient strategies.
- Prioritizing genes involved in critical biological processes is essential for maximizing research quality.
Purpose of the Study:
- To propose a method for identifying protein products of actively transcribed genes.
- To facilitate the direct sequencing of genes linked to essential biological functions.
Main Methods:
- Utilizing two-dimensional acrylamide gel electrophoresis to separate and identify protein products.
- Employing powerful computer software for analysis of gel data.
- Scaling gels to obtain sufficient protein for direct amino acid sequencing.
Main Results:
- Demonstrated a method to link protein sequences back to their corresponding genes.
- Enabled the identification of protein products from crucial genes.
Conclusions:
- The proposed method enhances the efficiency and quality of gene sequencing efforts.
- This approach provides a direct link between protein analysis and genetic information, aiding genome initiatives.