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Updated: May 16, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis
Giuliano Garofolo1, Massimo Ancora, Elisabetta Di Giannatale
1Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale - National and OIE Reference Laboratory for Brucellosis, Italy. g.garofolo@izs.it
Abstract:
The Multi Locus Variable Number Tandem Repeat Analysis 16 loci panel (MLVA-16), involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp., used also for the Brucella international online database. We describe an alternative, reliable, high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.

