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Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

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Related Experiment Video

Updated: May 16, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

The spindle assembly checkpoint.

Pablo Lara-Gonzalez1, Frederick G Westhorpe, Stephen S Taylor

  • 1Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.

Current Biology : CB
|November 24, 2012
PubMed
Summary
This summary is machine-generated.

The spindle assembly checkpoint ensures genome stability during cell division by delaying mitosis and meiosis until chromosomes are correctly attached to the spindle. This review details how the checkpoint signal is generated, blocks cell cycle progression, and is ultimately turned off.

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Last Updated: May 16, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • The spindle assembly checkpoint (SAC) is crucial for maintaining genome stability.
  • SAC delays cell division to ensure accurate chromosome segregation.
  • Kinetochore attachment to the spindle is essential for SAC inactivation.

Purpose of the Study:

  • To review recent advancements in understanding the SAC.
  • To elucidate the mechanisms of SAC signal generation.
  • To explain how SAC blocks cell cycle progression and is extinguished.

Main Methods:

  • Literature review of recent research on the spindle assembly checkpoint.
  • Analysis of molecular mechanisms underlying SAC signaling.
  • Examination of cell cycle regulation in response to kinetochore attachment.

Main Results:

  • SAC activation occurs when kinetochores are improperly attached to spindle microtubules.
  • Checkpoint signaling blocks cell cycle progression, preventing premature anaphase.
  • Stable kinetochore attachment leads to SAC inactivation and cell division.

Conclusions:

  • The SAC network is a complex system ensuring fidelity of chromosome segregation.
  • Understanding SAC regulation is key to comprehending genome stability.
  • Recent progress has shed light on the dynamic nature of SAC signaling and its timely inactivation.