Related Experiment Videos
Effect of calcium ion on the dimerization of alpha-chymotrypsin
1Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Biochimica Et Biophysica Acta
|March 29, 1990
Abstract:
Results of a sedimentation equilibrium study of the inhibitory effect of calcium ion on the dimerization of alpha-chymotrypsin (pH 3.9, I 0.2) are used to establish that the phenomenon does not reflect increased electrostatic repulsion between Ca2(+)-saturated enzyme molecules, but rather the displacement of the monomer-dimer equilibrium by the specific, weak interaction of metal ion with a single site on monomeric enzyme.