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Rapid detection of Campylobacter antigen by enzyme immunoassay leads to increased positivity rates

Carmen L Giltner1, Sandra Saeki, April M Bobenchik

  • 1David Geffen School of Medicine, Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA.

Journal of Clinical Microbiology
|November 24, 2012
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.