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Polymorphism of CD36 gene, carbohydrate metabolism and plasma CD36 concentration in obese children. A preliminary

Monika E Rać1, Beata Krupa, Barbara Garanty-Bogacka

  • 1Department of Biochemistry and Medical Chemistry Pomeranian Medical University, 70-111 Szczecin, Poland. carmon12@gmail.com

Postepy Higieny I Medycyny Doswiadczalnej (Online)
|November 24, 2012
PubMed

Insights

CD36 gene variations were not linked to carbohydrate metabolism issues in obese children. Further research is needed to understand the functional roles of these CD36 polymorphisms.

Area of Science:

  • Genetics
  • Metabolic Disorders
  • Pediatrics

Background:

  • CD36 is implicated in oxidized LDL removal, protein glycation, insulin resistance, and diabetic complications.
  • Previous studies suggest a link between CD36 gene mutations and reduced macrophage expression in obese, hyperglycemic individuals.
  • Investigating CD36 gene polymorphism in obese children is crucial for understanding its role in metabolic health.

Purpose of the Study:

  • To investigate the association between CD36 gene polymorphism and carbohydrate metabolism disturbances in obese children.
  • To examine the relationship between CD36 gene variants and plasma soluble CD36 concentrations in this population.

Main Methods:

  • The study included 60 obese children (10-15 years) and 30 controls.
  • Measurements included glycated hemoglobin, BMI, blood pressure, and oral glucose tolerance tests with insulin measurements.
  • CD36 gene exons 4-6 were analyzed using DHPLC and sequencing, with plasma CD36 levels measured by ELISA.

Main Results:

  • Two intronic alterations (rs3173798, rs3211892), one nonsynonymous substitution (rs183461468), and two synonymous transitions (rs5956, rs141680676) in the CD36 gene were identified.
  • No significant differences were observed in biochemical or morphometric parameters across different genotype groups.

Conclusions:

  • CD36 gene polymorphisms in the studied region are not associated with carbohydrate metabolism disturbances or soluble CD36 levels in obese children.
  • Further investigation is required to elucidate the functional significance of these CD36 polymorphisms.
Abstract

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