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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

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Related Experiment Video

Updated: May 16, 2026

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

Published on: February 25, 2013

Gene expression profiling revealed specific spermatogonial stem cell genes in mouse.

Lele Yang¹, Wei Wu, Huayu Qi

¹Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.

Genesis (New York, N.Y. : 2000)

|November 24, 2012

Summary

Spermatogonial stem cells (SSCs) self-renew and differentiate to produce sperm. Gene expression profiling reveals distinct molecular signatures in SSCs compared to other germ cells, highlighting key regulators of stem cell function.

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Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

Area of Science:

Reproductive Biology
Stem Cell Biology
Genomics

Background:

Mammalian spermatogenesis relies on spermatogonial stem cells (SSCs) for continuous sperm production.
SSCs possess self-renewal and differentiation capabilities, crucial for maintaining pluripotency.
Regulation of SSCs involves both extrinsic somatic cell factors and intrinsic gene expression programs.

Purpose of the Study:

To compare global gene expression profiles of mouse gonocytes, SSCs, and differentiated cells.
To identify distinctive genes and functional pathways associated with SSCs.
To explore molecular mechanisms underlying SSC self-renewal and differentiation.

Main Methods:

Purification of mouse gonocytes and SSCs using fluorescence-activated cell sorting (FACS).
Microarray analysis to determine global gene expression profiles.
In situ hybridization and RT-PCR to confirm gene expression patterns.

Main Results:

Distinctive gene expression profiles were identified for gonocytes, SSCs, and differentiated cells.
Gonocytes and SSCs showed enrichment of genes involved in gene expression regulation and epigenetic modifications.
Differentiated and somatic cells were enriched with genes related to diverse cellular activities.

Conclusions:

Comparative gene expression profiling provides a valuable resource for discovering genes involved in SSC regulation.
The study opens new avenues for investigating the molecular basis of SSC self-renewal and differentiation.
Identification of novel SSC-specific genes offers potential targets for future research.