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Related Experiment Video

Updated: May 16, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

QuickTox kit for QuickScan aflatoxin.

Cheryl Bailey¹, Alan H Davis

¹EnviroLogix Inc., 500 Riverside Industrial Pkwy, Portland, ME 04103, USA.

Journal of AOAC International

|November 27, 2012

Summary

This summary is machine-generated.

The QuickTox Kit for QuickScan Aflatoxin provides a reliable, user-friendly method for quantifying total aflatoxins in corn. This lateral flow assay meets all performance criteria for accuracy and stability.

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Published on: December 21, 2015

Area of Science:

Agricultural Science
Analytical Chemistry
Food Safety

Background:

Aflatoxins are toxic secondary metabolites produced by certain fungi, posing significant health risks to humans and animals.
Accurate quantification of total aflatoxins in food commodities like corn is crucial for regulatory compliance and consumer safety.
Existing methods for aflatoxin detection can be time-consuming or require specialized equipment, necessitating rapid and reliable alternatives.

Purpose of the Study:

To evaluate the performance of the QuickTox Kit for QuickScan Aflatoxin assay for the quantitative determination of total aflatoxins in corn.
To assess the assay's reliability, accuracy, and robustness against established performance criteria.

Main Methods:

The QuickTox Kit utilizes lateral flow technology combined with a reader-based system for quantitative analysis.
Performance was evaluated through internal and independent laboratory studies, including assessments of linearity, selectivity, matrix effects, robustness, and stability.
Corn samples naturally contaminated with aflatoxins were used to validate the assay's performance.

Main Results:

The assay demonstrated a linear dose response over the 0-100 ppb range (R2 > 0.98).
Relative Standard Deviation of the repeatability (RSDr) values ranged from 4.7% to 17.7%.
The assay detected the four major aflatoxin types in corn, with highest sensitivity for aflatoxin B1, and was unaffected by other common mycotoxins or variations in assay timing, temperature, and sample volume.

Conclusions:

The QuickTox Kit for QuickScan Aflatoxin assay is a user-friendly and reliable method for the quantitative determination of total aflatoxins in corn.
The assay meets predefined acceptance criteria for linearity, selectivity, matrix effects, robustness, and stability.
This method offers a dependable solution for rapid aflatoxin testing in agricultural products.