Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...

Sputum Studies II: Culture and Sensitivity

Sputum Studies II: Culture and Sensitivity

Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

Pulmonary Tuberculosis II

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...

Pulmonary Tuberculosis IV

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

Pulmonary Tuberculosis I

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...

Pulmonary Tuberculosis III

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Studies on light catalysis oxidation degradation of Malachite Green by Photo-Fenton reagent].

Guang pu xue yu guang pu fen xi = Guang pu·2007

Same author

Alternative conformations of the archaeal Nop56/58-fibrillarin complex imply flexibility in box C/D RNPs.

Journal of molecular biology·2007

Same author

Validation of nonnested and real-time PCR for diagnosis of sheep-associated malignant catarrhal fever in clinical samples.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2007

Same author

Room temperature fabrication of porous ZnO photoelectrodes for flexible dye-sensitized solar cells.

Chemical communications (Cambridge, England)·2007

Same author

Influence of medication choice and comorbid diabetes: the cost of bipolar disorder in a privately insured US population.

Social psychiatry and psychiatric epidemiology·2007

Same author

Mechanistic studies of the long chain acyl-CoA synthetase Faa1p from Saccharomyces cerevisiae.

Biochimica et biophysica acta·2007

Search research articles

Related Experiment Video

Updated: May 16, 2026

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Complex sputum microbial composition in patients with pulmonary tuberculosis.

Zelin Cui¹, Yuhua Zhou, Hong Li

¹Department of Medical Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China.

BMC Microbiology

|November 27, 2012

Summary

Pulmonary tuberculosis patients exhibit a more diverse sputum microbiome, including unique foreign bacteria, compared to healthy individuals. These microbial differences may influence tuberculosis development and progression.

More Related Videos

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

Published on: May 8, 2017

Related Experiment Videos

Last Updated: May 16, 2026

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

Published on: May 8, 2017

Area of Science:

Microbiology
Pulmonology
Genomics

Background:

The human microbiome plays a role in chronic diseases.
Investigating the sputum microbiome in pulmonary tuberculosis is crucial.

Purpose of the Study:

To explore bacterial communities in the sputum of pulmonary tuberculosis patients.
To compare the sputum microbiome of tuberculosis patients with healthy individuals.

Main Methods:

DNA extraction from sputum samples of 31 tuberculosis patients and 24 healthy controls.
16S rRNA gene sequencing (V3 hyper-variable regions) using Roche 454 FLX.
Bioinformatic analysis for microbial community profiling.

Main Results:

Sputum microbiota diversity is significantly higher in pulmonary tuberculosis patients (p<0.05).
24 bacterial phyla identified in patients versus 17 in healthy controls.
Unique foreign bacteria, including Stenotrophomonas and Pseudomonas, were prevalent in tuberculosis patients.

Conclusions:

The respiratory tract microbial composition is more complex in pulmonary tuberculosis patients.
The presence and role of foreign bacteria in tuberculosis pathogenesis warrant clinical consideration.
Microbiome analysis offers insights into tuberculosis disease mechanisms.