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Chromosome analysis of cryopreserved cells
T S McConnell1, L M Cordova, N A Baczek
1Department of Pathology, University of New Mexico Medical Center, Albuquerque 87131.
Cancer Genetics and Cytogenetics
|April 1, 1990
Summary
Cryopreservation of human leukemic cells for cytogenetic studies yielded successful chromosome analysis in about 50% of cases. While not a replacement for fresh samples, this method offers a viable alternative for specific research objectives.
Area of Science:
- Hematology
- Oncology
- Cytogenetics
Background:
- Cytogenetic analysis is crucial for diagnosing and understanding leukemias.
- Access to fresh patient samples for timely cytogenetic studies can be challenging.
- Cryopreservation offers a potential method for preserving biological samples for later analysis.
Purpose of the Study:
- To evaluate a technique for freezing and thawing human leukemic cells for cytogenetic analysis.
- To determine the feasibility and quality of chromosome studies from cryopreserved leukemic cells.
- To assess the utility of cryopreserved leukemic cells as an alternative to fresh material.
Main Methods:
- A technique for freezing and thawing human leukemic cells was developed and applied.
- Sixty-four harvests from 26 cryopreserved samples from 20 individuals were studied.
- Chromosome quality, metaphase yield, and banding were assessed.
Main Results:
- Chromosome quality was notably better in samples from patients with myeloid disorders.
- Successful chromosome studies were achieved in approximately 50% of the cryopreserved samples.
- The method resulted in a relatively low number of metaphases and mediocre chromosome spreading and banding.
Conclusions:
- Cryopreservation of leukemic cells is not a complete substitute for fresh material due to limitations in metaphase yield and chromosome quality.
- Chromosome analysis of cryopreserved leukemic cells can be a viable alternative when specific research objectives are defined.
- This technique may be particularly useful for specific patient groups, such as those with myeloid disturbances.