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Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Destabilization of Microtubules01:45

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...

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Related Experiment Video

Updated: May 16, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance.

Mark Mason1, Jennifer J Wanat2, Sandy Harper3

  • 1The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA; Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.

Structure (London, England : 1993)
|November 27, 2012
PubMed
Summary

Cdc13 protein dimerization is crucial for telomere stability. This OB2 domain homodimerization ensures proper assembly of the Cdc13, Stn1, and Ten1 (CST) complex for effective telomere capping.

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Last Updated: May 16, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
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Published on: August 30, 2024

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Cdc13 is vital for yeast telomere length regulation and genome stability.
  • It functions by capping telomeres and controlling telomerase access.

Purpose of the Study:

  • To investigate the structural and functional role of the Cdc13 OB2 domain.
  • To determine the importance of OB2 domain dimerization in Cdc13 function and telomere capping.

Main Methods:

  • Crystal structure determination of the Saccharomyces cerevisiae Cdc13 OB2 domain.
  • Biochemical assays to assess DNA binding, Stn1 binding, and dimerization.
  • Analysis of the impact of mutations on Cdc13-Stn1 association and telomere length.

Main Results:

  • The Cdc13 OB2 domain adopts an oligonucleotide-oligosaccharide binding fold (OB2) with long loops facilitating homodimerization.
  • The cdc13-1 mutation disrupts OB2 dimerization, affecting Cdc13-Stn1 association.
  • Disruption of OB2 dimerization leads to telomere length deregulation and temperature sensitivity.

Conclusions:

  • OB2 domain dimerization is essential for stable homodimerization of Cdc13.
  • Cdc13 OB2 dimerization is required for the proper assembly of the Cdc13, Stn1, Ten1 (CST) complex.
  • This dimerization is critical for productive telomere capping and maintaining genome stability.