Bioevaluation of Anisomeles indica extracts and their inhibitory effects on Helicobacter pylori-mediated inflammation

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Hsiu Man Lien1, Chyi Yann Wang, Hsiao Yun Chang

  • 1Department of Chemistry, Tunghai University, Taichung, Taiwan.

Journal of Ethnopharmacology
|November 27, 2012
PubMed
Abstract

Insights

Anisomeles indica extracts, rich in ovatodiolide (OVT), show potent activity against Helicobacter pylori. These extracts also reduce inflammation, offering a promising therapeutic option for H. pylori infections.

Area of Science:

  • Pharmacology
  • Microbiology
  • Natural Products Chemistry

Background:

  • Helicobacter pylori infection is a major cause of gastric disorders, with rising antibiotic resistance globally.
  • Anisomeles indica and its compound ovatodiolide (OVT) exhibit bactericidal effects against H. pylori.

Purpose of the Study:

  • To develop Anisomeles indica extracts rich in OVT.
  • To evaluate the bactericidal efficacy of these extracts against H. pylori.
  • To assess the anti-inflammatory properties of the extracts in H. pylori-infected models.

Main Methods:

  • Extraction of Anisomeles indica using varying ethanol concentrations.
  • Quantification of OVT content via High-Performance Liquid Chromatography (HPLC).
  • In vitro assessment of antibacterial activity and inhibition of inflammatory markers (NF-κB, IL-8, iNOS, NO, TNF-α).

Main Results:

  • 50% and 95% ethanol extracts demonstrated high OVT content and significant anti-H. pylori activity.
  • The 95% ethanol extract suppressed H. pylori-induced NF-κB activation and IL-8 secretion in gastric cells.
  • The extract also inhibited LPS-induced iNOS, NO, and TNF-α production in macrophages.

Conclusions:

  • Anisomeles indica ethanol extracts containing OVT are effective against H. pylori.
  • These extracts show potential for mitigating H. pylori-associated inflammation.
  • The findings suggest a potent, economical therapeutic strategy for H. pylori infection.

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