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Related Experiment Videos

A microalbuminuria assay using bromphenol blue.

K Jung1, E Nickel, M Pergande

  • 1Department of Experimental Organ Transplantation, University Hospital Charité, Humboldt University Berlin, GDR.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|February 28, 1990
PubMed
Summary

This study presents a simple, cost-effective microalbuminuria assay using bromphenol blue/glycine. The assay is precise, accurate, and suitable for non-specialist labs, offering a reliable method for detecting microalbuminuria.

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Area of Science:

  • Clinical Chemistry
  • Biochemical Assays
  • Urine Analysis

Background:

  • Microalbuminuria is an early indicator of kidney disease, particularly in diabetic patients.
  • Accurate and accessible diagnostic tools are crucial for early detection and management.
  • Existing methods can be complex or expensive, limiting their widespread use.

Purpose of the Study:

  • To develop and validate a simple, cost-effective assay for quantifying microalbuminuria.
  • To establish the analytical performance characteristics of the new assay.
  • To assess its suitability for routine laboratory use.

Main Methods:

  • Urine samples were prepared using gel filtration on Sephadex G-50 minicolumns.
  • A bromphenol blue/glycine reagent was used for colorimetric detection of albumin.

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  • Absorbance was measured at 610 nm, with results correlated against radial immunodiffusion.

    • Main Results:

    • The assay demonstrated a detection limit of 3 mg/l.
    • Excellent precision was observed (0.5-4.1% CV) for borderline and raised albumin levels.
    • High recovery (98.7 +/- 2.5%) and strong correlation (r=0.987) with radial immunodiffusion were achieved.
    • Reagent cost was minimal (approx. 5 cents per test).

    Conclusions:

    • The described microalbuminuria assay is simple, sensitive, precise, and accurate.
    • Its low cost and ease of use make it ideal for non-specialist laboratories.
    • This assay provides a valuable tool for early detection of kidney damage.