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Updated: May 16, 2026

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

Authentic human basic fibroblast growth factor produced by secretion in Bacillus subtilis.

Keith W Y Kwong¹, K L Ng, C C Lam

¹Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

Applied Microbiology and Biotechnology

|November 27, 2012

Summary

Engineered Bacillus subtilis successfully secreted human basic fibroblast growth factor (hbFGF), achieving a high yield of 40 mg/L. This breakthrough offers a cost-effective method for producing this valuable protein for medical applications.

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Area of Science:

Microbial biotechnology
Protein expression
Biopharmaceutical production

Background:

Bacillus subtilis is a recognized host for heterologous protein production.
Commercial application of B. subtilis for medical proteins remains limited.
High cost of native hbFGF hinders medical and skincare applications.

Purpose of the Study:

To develop an engineered Bacillus subtilis system for efficient hbFGF secretion.
To establish a cost-effective production method for human basic fibroblast growth factor (hbFGF).

Main Methods:

Utilized an engineered Bacillus subtilis strain.
Implemented a novel cell-wall destabilization protocol.
Quantified secreted hbFGF yield and confirmed its bioactivity and sequence identity.

Main Results:

Achieved a significant yield of 40 mg/L of secreted hbFGF.
Confirmed the secreted hbFGF exhibited potent bioactivity.
Verified the protein sequence was identical to mature native hbFGF (Mat-hbFGF).

Conclusions:

The engineered B. subtilis system provides a viable platform for hbFGF production.
This method could significantly reduce the cost of Mat-hbFGF for therapeutic and cosmetic use.
Paves the way for cost-effective biopharmaceutical manufacturing.