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Related Experiment Video

Updated: Jan 9, 2026

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time
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Optical and opto-acoustic imaging.

Vasilis Ntziachristos1, Daniel Razansky

  • 1Institute for Biological and Medical Imaging,Technische Universität München and Helmholtz Zentrum München, Munich, Germany. v.ntziachristos@tum.de

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Summary

Optical imaging advances enable deep tissue visualization in vivo. Multispectral optoacoustic tomography provides high-resolution, real-time 3D imaging for preclinical research and clinical applications.

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Area of Science:

  • Biomedical imaging
  • Optical tomography
  • Molecular imaging

Background:

  • Optical imaging has been crucial for biological discovery for over 400 years.
  • In vivo staining methods (bioluminescence, fluorescent probes, nanoparticles) enable molecular-level interrogation of biological processes.
  • Advanced optical tomography provides volumetric visualization of biomarkers in small animals, overcoming limitations of traditional microscopy in deep tissues.

Purpose of the Study:

  • To highlight the advancements in optical imaging technologies for in vivo studies.
  • To introduce biomedical optoacoustics as a solution for deep tissue imaging limitations.
  • To explain the capabilities of multispectral optoacoustic tomography (MSOT) for preclinical and clinical applications.

Main Methods:

  • Development of advanced optical tomographic imaging technologies.
  • Utilizing in vivo staining methods like bioluminescence and fluorescent probes.
  • Employing biomedical optoacoustics, specifically multispectral optoacoustic tomography (MSOT).

Main Results:

  • MSOT overcomes light scattering limitations in deep tissues.
  • MSOT provides high-resolution, real-time, 3D volumetric imaging of deep living tissues.
  • MSOT enables "hearing color" by detecting sound vibrations from selective light absorption at multiple wavelengths.

Conclusions:

  • Multispectral optoacoustic tomography (MSOT) offers powerful capabilities for high-resolution, deep-tissue imaging.
  • MSOT is a significant advancement for preclinical screening in animal models.
  • The technology is expected to impact clinical decision-making in the future.