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Updated: May 16, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
On programmed ribosomal frameshifting: the alternative proteomes
1MRC Laboratory for Molecular Cell Biology, Translational Research Resource Centre, University College London London, UK.
Abstract:
FRAMESHIFTING RESULTS FROM TWO MAIN MECHANISMS: genomic insertions or deletions (indels) or programmed ribosomal frameshifting. Whereas indels can disrupt normal protein function, programmed ribosomal frameshifting can result in dual-coding genes, each of which can produce multiple functional products. Here, I summarize technical advances that have made it possible to identify programmed ribosomal frameshifting events in a systematic way. The results of these studies suggest that such frameshifting occurs in all genomes, and I will discuss methods that could help characterize the resulting alternative proteomes.
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