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Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
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Updated: May 16, 2026

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
07:10

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

Budding behavior of multi-component vesicles.

Jianfeng Li1, Hongdong Zhang, Feng Qiu

  • 1The State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China. lijf@fudan.edu.cn

The Journal of Physical Chemistry. B
|November 28, 2012
PubMed
Summary
This summary is machine-generated.

The budding number of multicomponent vesicles is primarily governed by component-specific elastic properties, while budding types are influenced by line tension. A phase diagram illustrates budding behaviors, validated by simulations.

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Area of Science:

  • Biophysics
  • Materials Science
  • Computational Biology

Background:

  • Multicomponent vesicles exhibit complex budding behaviors.
  • Line tension and component-dependent elastic properties are key factors influencing vesicle dynamics.

Purpose of the Study:

  • To investigate the distinct roles of line tension and elastic properties on the budding of two-component vesicles.
  • To establish a phase diagram for vesicle budding based on these parameters.

Main Methods:

  • Utilized a spherical-cap model to analyze vesicle budding.
  • Employed computer simulations to validate theoretical findings and explore model extensions.

Main Results:

  • Budding number is predominantly determined by component-dependent elastic properties.
  • Budding types are primarily mediated by line tensions at domain boundaries.
  • A phase diagram was generated, delineating regions of partial budding, partial bud-off, and total bud-off.

Conclusions:

  • Component-dependent elastic properties and line tensions have distinct, crucial roles in multicomponent vesicle budding.
  • The spherical-cap model provides a valid framework for understanding these phenomena.
  • Developed simulation techniques are adaptable for broader applications in multicomponent vesicle research.