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Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
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Published on: August 25, 2018

Multiple tick-associated bacteria in Ixodes ricinus from Slovakia.

Geetha Subramanian1, Zuzana Sekeyova, Didier Raoult

  • 1Aix Marseille Université, Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, Inserm 1095, WHO Collaborative Center for Rickettsioses and Other Arthropod Borne Bacterial Diseases, 27 bd Jean Moulin, 13385 Marseille Cedex 5, France.

Ticks and Tick-Borne Diseases
|November 28, 2012
PubMed
Summary

Eighty Ixodes ricinus ticks from Slovakia were analyzed for bacterial pathogens. While some common tick-borne bacteria were detected, a significant portion of ticks harbored other bacteria, some with unknown human pathogenicity.

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Area of Science:

  • Veterinary Entomology
  • Microbiology
  • Molecular Diagnostics

Background:

  • Ixodes ricinus ticks are significant vectors of various bacterial pathogens.
  • Understanding the microbial communities within tick populations is crucial for public health surveillance.

Purpose of the Study:

  • To investigate the prevalence of pathogenic and endosymbiotic bacteria in Ixodes ricinus ticks from Rovinka forest, Slovakia.
  • To identify specific bacterial species and their distribution within tick life stages.

Main Methods:

  • Real-time and routine single-step PCR techniques were employed.
  • Eighty Ixodes ricinus tick samples were collected and analyzed.
  • Detection of targeted bacterial DNA was performed.

Main Results:

  • Pathogenic bacteria detected included Anaplasma phagocytophilum (8%), Rickettsia helvetica (11%), and various Borrelia species (1-9%).
  • Other bacteria identified were Arsenophonus nasoniae (25%), Wolbachia aff. pipientis (14%), and Candidatus Midichloria mitochondrii (4%).
  • A. nasoniae was prevalent in nymphs, and Ehrlichia muris in adults; 65% of ticks carried at least one bacterium, 16% carried multiple.

Conclusions:

  • Ixodes ricinus ticks in Rovinka forest harbor a diverse range of bacteria, including known human pathogens and others with uncertain pathogenicity.
  • The findings highlight the complex microbial ecology of ticks and underscore the need for continued monitoring of tick-borne agents.