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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Label-free aptasensor for thrombin determination based on the nanostructured phenazine mediator
Gennady A Evtugyn1, Veronika B Kostyleva, Anna V Porfireva
1Analytical Chemistry Department of Kazan Federal University, 18 Kremlevskaya Street, Kazan 420008, Russian Federation. Gennady.Evtugyn@ksu.ru
Talanta
|November 28, 2012
Summary
New aptasensors utilizing DNA aptamers and thiacalixarenes detect thrombin. These biosensors show high sensitivity and specificity, with potential applications in diagnostics.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Materials Science
Background:
- Thrombin is a key biomarker in hemostasis and thrombosis.
- Development of sensitive and specific biosensors for thrombin detection is crucial for clinical diagnostics.
- Aptasensors offer advantages due to their stability and specificity.
Purpose of the Study:
- To develop novel aptasensors for thrombin detection.
- To investigate the role of thiacalixarene configuration in aptasensor performance.
- To optimize the biorecognition layer for enhanced signal transduction.
Main Methods:
- Fabrication of aptasensors using DNA aptamers and functionalized thiacalix[4]arenes.
- Surface characterization and optimization using Atomic Force Microscopy (AFM) and Electrochemical Impedance Spectroscopy (EIS).
- Electrochemical detection of thrombin based on the interaction with Neutral Red (NR) mediator.
Main Results:
- The developed aptasensors successfully detected thrombin in the range of 1 nM to 1 μM.
- A low detection limit of 0.05-0.5 nM was achieved.
- The aptasensor demonstrated high specificity, with no interference from a 1000-fold excess of bovine serum albumin.
- Thiacalixarene configuration influenced aptasensor sensitivity.
Conclusions:
- Novel aptasensors based on DNA aptamers and thiacalixarenes are effective for thrombin detection.
- The interaction mechanism involves suppression of electron hopping by thrombin binding.
- The study highlights the potential of these aptasensors for sensitive and specific thrombin quantification.
