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A Mr 80K hepatocyte surface protein(s) interacts with basement membrane components.

B Clément1, Y Yamada

  • 1Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.

Experimental Cell Research
|April 1, 1990
PubMed
Summary

Researchers identified an 80,000-Dalton (Mr 80K) cell surface protein on rat hepatocytes that binds to key basement membrane components like collagen IV, heparan sulfate proteoglycan, and laminin, suggesting a role in cell adhesion.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Proteomics

Background:

  • Hepatocytes interact with the extracellular matrix, particularly the basement membrane.
  • Understanding these interactions is crucial for liver function and regeneration.

Purpose of the Study:

  • To identify and characterize cell surface proteins on adult rat hepatocytes that bind to basement membrane components.
  • To investigate the role of these proteins in hepatocyte-basement membrane interactions.

Main Methods:

  • Cell surface iodination of isolated rat hepatocytes.
  • Affinity chromatography using purified collagen IV, heparan sulfate proteoglycan, and laminin.
  • Analysis of eluted proteins by SDS-PAGE and two-dimensional gel electrophoresis.

Main Results:

  • An 80,000-Dalton (Mr 80K) protein was identified that binds to collagen IV.
  • Mr 80K and 38K proteins were eluted from a heparan sulfate proteoglycan column.
  • Several proteins, including Mr 80K, bound to a laminin A chain-derived peptide column.
  • The Mr 80K proteins exhibited similar isoelectric points (pI 8-9) across different binding experiments.

Conclusions:

  • A Mr 80K cell surface protein on rat hepatocytes binds multiple basement membrane components.
  • This Mr 80K protein likely plays a significant role in mediating hepatocyte adhesion to the basement membrane.