Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane
Jose L Garcia-Cordero1, Sebastian J Maerkl
1Laboratory of Biological Network Characterization, Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne, Station 17, CH-1015, Lausanne, Switzerland.
Chemical Communications (Cambridge, England)
|November 28, 2012
Abstract:
We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.
