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Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

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Published on: May 10, 2020

Implication of hepatic stem cells in functional liver repopulation.

Bruno Christ¹, Sandra Pelz

¹Translational Centre for Regenerative Medicine-TRM, University of Leipzig, Philipp-Rosenthal-Straße 55, D-04103 Leipzig, Germany. bruno.christ@medizin.uni-leipzig.de

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|November 28, 2012

Summary

This review examines hepatic stem cell candidates, evaluating their potential for liver regeneration and repair. It also explores how adult stem cells from other tissues could generate functional hepatocytes for treating liver diseases.

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Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations

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Published on: October 26, 2011

Area of Science:

Hepatology and Stem Cell Biology
Regenerative Medicine

Background:

The liver possesses significant regenerative capacity following injury, utilizing hepatocytes or liver progenitor cells.
The existence and role of hepatic stem cells in liver maintenance and regeneration remain subjects of ongoing scientific debate.

Purpose of the Study:

To critically evaluate potential hepatic stem cell candidates based on established stem cell criteria.
To explore the therapeutic potential of adult stem cells from non-hepatic sources for generating functional hepatocytes.

Main Methods:

Review of existing literature on liver regeneration and stem cell biology.
Analysis of stem cell criteria including self-renewal, multipotency, and serial transplantability.
Examination of lineage tracing and immunohistochemical studies for progenitor identification.
Consideration of flow cytometry data on liver stem cell heterogeneity.

Main Results:

Various cell populations in fetal and adult livers meet stem cell criteria under normal and injury conditions.
Evidence suggests heterogeneity within the liver stem cell population, with distinct molecular signatures.
Adult stem cells from bone marrow, cord blood, and adipose tissue can differentiate into hepatocyte-like cells.

Conclusions:

Hepatic stem cell candidates exist and warrant further investigation based on defined criteria.
The potential for non-hepatic adult stem cells to become functional hepatocytes offers promising therapeutic avenues for end-stage liver diseases, potentially reducing the need for whole organ transplantation.