Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photodynamically active AIE-functionalised casein nanofibers for antibacterial wound management in epidermolysis bullosa.

Journal of materials chemistry. B·2026
Same author

Targeting hemozoin detoxification in Plasmodium falciparum: could a chlorophyll derivative act as a tetrapyrrole mimic to disrupt hemozoin formation in infected erythrocytes?

MedScience·2026
Same author

Multifunctional Oregano-Derived Plasma Polymer Coatings for Wound Healing Applications: An In Vitro Study.

International wound journal·2026
Same author

Antibacterial ZnO nanoparticle embedded polycaprolactone-polyhydroxybutyrate membranes for wound healing.

Journal of materials science. Materials in medicine·2026
Same author

Overcoming challenges in cartilage regeneration: The role of chondrogenic inducers.

Bioengineering & translational medicine·2026
Same author

Cortistatin antagonizes Piezo1-STING axis and facilitates mitochondrial homeostasis of keratinocytes by attenuating AGEs accumulation in diabetic ulcers.

Cell death and differentiation·2026

Related Experiment Video

Updated: May 16, 2026

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
11:39

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

Surface bound amine functional group density influences embryonic stem cell maintenance.

Frances Harding1, Renee Goreham, Robert Short

  • 1University of South Australia, Adelaide, SA, Australia.

Advanced Healthcare Materials
|November 28, 2012
PubMed
Summary
This summary is machine-generated.

Embryonic stem cells (ESCs) maintain pluripotency on specific plasma polymer gradients. Optimal conditions for stem cell markers alkaline phosphatase (AP) and Oct4 were identified on octadiene-rich surfaces.

More Related Videos

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Related Experiment Videos

Last Updated: May 16, 2026

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
11:39

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Surface Chemistry

Background:

  • Gradient surfaces enable the study of cell-surface interactions.
  • Plasma polymers offer tunable surface properties for biological applications.

Purpose of the Study:

  • To investigate embryonic stem cell (ES cell) colony response to plasma polymer gradient surfaces.
  • To identify optimal surface chemistries for retaining pluripotency in ES cells.

Main Methods:

  • Fabrication of plasma polymer gradients using allylamine (AA) and octadiene (OD).
  • Culture of ES cell colonies on the gradient surfaces.
  • Analysis of stem cell marker expression (alkaline phosphatase and Oct4) and colony morphology.
  • Assessment of cell adhesion and actin polymerization.

Main Results:

  • Stem cell markers (AP and Oct4) were best retained on octadiene (OD) rich regions of the gradient.
  • Optimal retention of Oct4 and AP expression occurred at a nitrogen-to-carbon (N/C) ratio < 0.04, distinct from maximum cell adhesion.
  • Compact, multilayered colonies with strong F-actin staining correlated with decreased N/C ratio.
  • Disruption of actin polymerization led to monolayer formation and decreased marker expression, with partial rescue on OD-rich regions.

Conclusions:

  • Plasma polymer gradients can be used to optimize surface conditions for maintaining ES cell pluripotency.
  • Surface chemistry, particularly nitrogen content, significantly influences stem cell marker expression and colony morphology.
  • Actin dynamics play a crucial role in maintaining the 3D structure and pluripotency of ES cell colonies on these surfaces.