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Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

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Related Experiment Video

Updated: May 16, 2026

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

Efficient cell reprogramming using bioengineered surfaces.

David Horna1, Juan Carlos Ramírez, Anna Cifuentes

  • 1Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.

Advanced Healthcare Materials
|November 28, 2012
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Summary

Researchers developed a novel cell reprogramming method using modified surfaces. This technique efficiently reprograms somatic cells into pluripotent cells, offering a new approach for regenerative medicine.

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Last Updated: May 16, 2026

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Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Surface Chemistry

Background:

  • Cell reprogramming is crucial for regenerative medicine and disease modeling.
  • Current methods for cell reprogramming often face challenges with efficiency and scalability.
  • Developing novel surfaces for efficient cell manipulation is an active area of research.

Purpose of the Study:

  • To develop a novel method for efficient cell reprogramming.
  • To create specialized surfaces for immobilizing nucleic acid transfer vectors.
  • To investigate the potential of these surfaces in generating pluripotent cells.

Main Methods:

  • Utilizing pentafluorophenyl methacrylate (PFM)-modified surfaces created via Ar plasma treatment.
  • Immobilizing nucleic acid transfer vectors, such as lentiviral particles with free amino groups, onto PFM-modified surfaces.
  • Applying these modified surfaces, termed cell reprogramming surfaces (CRS), for somatic cell reprogramming.

Main Results:

  • Successfully developed PFM-grafted surfaces for vector immobilization.
  • Demonstrated high efficiencies in reprogramming murine somatic cells into pluripotent cells using CRS.
  • Established a novel and effective platform for cell reprogramming.

Conclusions:

  • The developed cell reprogramming surfaces (CRS) offer a highly efficient method for generating pluripotent cells.
  • This novel technique advances cell reprogramming strategies with potential applications in regenerative medicine.
  • The PFM-modified surfaces provide a robust platform for nucleic acid transfer vector immobilization.