Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multistage microrobots with pH-responsive release of platelet membrane-coated nanoparticles.

Science advances·2026
Same author

A Dural Extracellular Matrix Hydrogel with Neural Stem Cells Improves Recovery from Traumatic Brain Injury in Mice.

ACS biomaterials science & engineering·2026
Same author

BODIPY Photocage-Based Injectable Hydrogel for Light-Controlled Nanoparticle Release.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Bionano Interface Optimization for Rational Lateral Flow Assay Development.

ACS nano·2026
Same author

Image-guided optimization of regenerative graft attachment to the heart.

Biomaterials·2026
Same author

Towards intelligent and miniaturized drug delivery devices.

Nature·2026

Related Experiment Video

Updated: May 16, 2026

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Published on: October 7, 2015

Latent TGF-β hydrogels for cartilage tissue engineering.

Elsie S Place1, Rekha Nair, Helena N Chia

  • 1Department of Materials, Imperial College, London SW7 2AZ, UK.

Advanced Healthcare Materials
|November 28, 2012
PubMed
Summary

This study presents a novel biomimetic delivery method for transforming growth factor beta (TGF-β). By tethering the small latent complex (SLC) to a hyaluronic acid hydrogel, researchers enhanced cartilage formation in vitro.

More Related Videos

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels
08:34

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Related Experiment Videos

Last Updated: May 16, 2026

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Published on: October 7, 2015

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels
08:34

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Transforming growth factor beta (TGF-β) is crucial for tissue regeneration but requires controlled delivery.
  • The small latent complex (SLC) form of TGF-β is inactive until cellular modification, offering a potential delivery advantage.
  • Hyaluronic acid hydrogels are biocompatible scaffolds with potential in tissue engineering.

Purpose of the Study:

  • To develop a biomimetic delivery strategy for TGF-β using a hyaluronic acid hydrogel scaffold.
  • To investigate the potential of tethered SLC to enhance chondrogenesis (cartilage formation) in vitro.
  • To create a system where TGF-β activation is cell-mediated.

Main Methods:

  • Tethering the small latent complex (SLC) of TGF-β to a hyaluronic acid hydrogel.
  • Utilizing the hydrogel scaffold for in vitro cell culture.
  • Assessing chondrogenesis through relevant biological assays.

Main Results:

  • Successful tethering of SLC to the hyaluronic acid hydrogel was achieved.
  • The biomimetic delivery system demonstrated enhanced in vitro chondrogenesis compared to controls.
  • Cell-mediated modification of the latent TGF-β complex was implied by the observed biological response.

Conclusions:

  • Tethering latent TGF-β (SLC) to hyaluronic acid hydrogels is a viable biomimetic delivery strategy.
  • This approach effectively promotes in vitro chondrogenesis.
  • Cell-controlled release and activation of TGF-β from hydrogel scaffolds show promise for regenerative medicine applications.