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An antithrombogenic citric acid-crosslinked gelatin with endothelialization activity
Motoki Inoue1, Makoto Sasaki, Asako Nakasu
1Biomaterials Unit, Nano-bio field, Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Advanced Healthcare Materials
|November 28, 2012
Summary
A novel gelatin matrix crosslinked with trisuccinimidyl citrate (TSC) shows excellent endothelialization and anti-platelet activity. This biocompatible material reduces inflammation and shows potential for blood-contacting biomedical devices.
Area of Science:
- Biomaterials Science
- Biomedical Engineering
- Tissue Engineering
Background:
- Developing biocompatible materials for medical devices is crucial.
- Existing materials often face challenges with blood compatibility and inflammation.
- Gelatin-based matrices offer promise but require effective crosslinking for stability and function.
Purpose of the Study:
- To create and characterize a novel citric acid-crosslinked gelatin matrix.
- To evaluate its endothelialization activity and anti-platelet properties.
- To assess its biocompatibility and potential for biomedical applications.
Main Methods:
- Preparation of trisuccinimidyl citrate (TSC)-crosslinked gelatin matrix.
- Endothelial cell culture and antithrombogenic activity testing.
- Bioluminescence imaging for biocompatibility and inflammation assessment.
- Physicochemical analysis, including carboxyl group and cell adhesion sequence evaluation.
Main Results:
- TSC-crosslinked gelatin promoted endothelial cell adhesion and growth, with optimal results at 20 mM TSC.
- Minimal platelet adhesion and fibrin formation were observed on TSC-crosslinked gelatin compared to glutaraldehyde-crosslinked gelatin.
- Bioluminescence imaging indicated a lower inflammatory response for TSC-crosslinked gelatin.
- High concentrations of arginine-glycine-aspartic acid sequences and increased negative charge contributed to improved biocompatibility.
Conclusions:
- TSC-crosslinked gelatin exhibits significant endothelialization and antithrombogenic properties.
- The matrix demonstrates superior biocompatibility and reduced inflammation compared to glutaraldehyde-crosslinked gelatin.
- This novel material holds considerable potential for use in blood-contacting biomedical devices like stents and artificial valves.
