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Platelet phospholipase C activity in salt-dependent hypertension
I Limon1, J Blanc, S Koutouzov
1Department of Pharmacology, Necker Hospital, Paris, France.
Hypertension (Dallas, Tex. : 1979)
|April 1, 1990
Summary
Enhanced phospholipase C activity in platelets is linked to hypertension. Salt-sensitive rats on high-salt diets showed increased platelet responsiveness, suggesting a role in blood pressure regulation.
Area of Science:
- Cardiovascular Physiology
- Biochemistry
- Hypertension Research
Background:
- Enhanced phospholipase C responsiveness is observed in spontaneously hypertensive rats and some essential hypertension patients.
- Platelet hyperreactivity to thrombin is implicated in hypertension, potentially linked to increased phospholipase C activity.
Purpose of the Study:
- To investigate the relationship between platelet phospholipase C activity and experimental hypertension models.
- To determine if altered phospholipase C activity contributes to hypertension in Dahl salt-sensitive rats.
Main Methods:
- Investigated platelet phospholipase C activity in Dahl salt-resistant (SR/Jr) and salt-sensitive (SS/Jr) rats on low and high NaCl diets.
- Measured thrombin-induced [32P]phosphatidic acid formation and P47 protein labeling in prelabeled platelets.
- Assessed platelet reactivity via thrombin-induced serotonin release.
Main Results:
- SS/Jr rats on a high NaCl diet exhibited significantly increased platelet phospholipase C activity, P47 labeling, and serotonin release compared to those on a low NaCl diet.
- A high NaCl diet did not alter phospholipase C activity in SR/Jr rats.
- DOCA-salt hypertensive rats showed normal platelet reactivity and phospholipase C responsiveness.
Conclusions:
- Increased platelet phospholipase C activity and reactivity in salt-sensitive rats on a high-salt diet suggest a link to salt-induced hypertension.
- This enzymatic abnormality may play a role in the hyperresponsiveness observed in certain hypertension models.