Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two distinct regions in micellar aggregates identified with pyrene-labeled dendrimers.

Science advances·2026
Same author

Tailoring κ/ι-hybrid carrageenans from red algae Chondracanthus chamissoi via alkaline pretreatment: characterization and potential applications in vanilla custard preparation and methylene blue removal.

International journal of biological macromolecules·2026
Same author

Phthalimide Derivatives as Anti-Inflammatory Agents: In Silico COX-2 Targeting and In Vitro Inhibition of PGE<sub>2</sub> Production.

Pharmaceutics·2026
Same author

Advancing Single-Walled Carbon Nanotubes toward Next-Generation Electronic Materials: The Critical Roles of Purity and Processability.

Small methods·2026
Same author

A Dual Luminescent and Chromogenic Pt-NCN Complex for the Detection of GTP and CTP in Aqueous Media.

Inorganic chemistry·2026
Same author

Cellulose-Reinforced Carbon Nanotube Buckypapers with Balanced Mechanical Strength and Conductivity.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: May 16, 2026

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

Studying pyrene-labeled macromolecules with the model-free analysis.

Michael A Fowler1, Jean Duhamel, Greg J Bahun

  • 1Institute of Polymer Research, Waterloo Institute of Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

The Journal of Physical Chemistry. B
|November 29, 2012
PubMed
Summary

Model-free analysis accurately probes macromolecule dynamics, including polymer ring closure and backbone flexibility. This versatile method, applied to pyrene-labeled polymers, offers a robust way to benchmark internal dynamics.

More Related Videos

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
11:01

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Published on: August 24, 2021

Related Experiment Videos

Last Updated: May 16, 2026

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
11:01

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Published on: August 24, 2021

Area of Science:

  • Polymer Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Understanding macromolecule internal dynamics is crucial for material properties.
  • Pyrene-labeled macromolecules are widely used to study polymer behavior.
  • Existing models for fluorescence decay analysis have limitations.

Purpose of the Study:

  • To apply and validate model-free (MF) analysis for probing internal dynamics of diverse pyrene-labeled macromolecules.
  • To compare MF analysis results with established models like Birks' scheme and the fluorescence blob model (FBM).
  • To establish a calibration curve for benchmarking macromolecule dynamics.

Main Methods:

  • Analysis of fluorescence decays from 32 pyrene-labeled macromolecules.
  • Application of model-free (MF) analysis to study polymer ring closure, backbone flexibility, and chain terminal mobility.
  • Comparison of MF analysis results with Birks' scheme and FBM.

Main Results:

  • MF analysis successfully probed various internal dynamics in pyrene-labeled polymers.
  • Good agreement was observed between MF analysis and Birks' scheme/FBM for excimer formation rate constants.
  • The first direct comparison of chain terminal dynamics in pyrene end-labeled dendrons was achieved.
  • A calibration curve for benchmarking macromolecule internal dynamics was developed.

Conclusions:

  • Model-free analysis is a versatile and robust method for studying internal dynamics of pyrene-labeled macromolecules.
  • MF analysis provides reliable data comparable to established methods.
  • This study establishes a new benchmark for analyzing macromolecule dynamics.