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Updated: May 16, 2026
A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
Development of an analytical approach for identification and quantification of 5-α-androst-16-en-3-one in human milk
Constanze Hartmann1, Fabienne Mayenzet, Jean-Paul Larcinese
1Department of Chemistry and Pharmacy, Food Chemistry, Emil Fischer Center, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
Abstract:
A method was developed for the quantification of 5-α-androst-16-en-3-one in human breast milk based on application of a stable isotope dilution assay using 5α-androst-16-en-3-one-6, 6-d(2). The procedure includes extraction of the human milk by hexane with subsequent clean-up of the obtained extract by gel permeation and silica gel column chromatography. The extracted samples were analyzed by gas chromatography-mass spectrometry. Using this method 5-α-androst-16-en-3-one could be identified and for the first time quantified in a concentration range of 26-155 ng/kg in human milk.
