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Updated: May 16, 2026

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Published on: June 23, 2023
Morphological operation-based bi-dimensional empirical mode decomposition for automatic background removal of fringe
Xiang Zhou1, Adrian Gh Podoleanu, Zhuangqun Yang
1State Key Laboratory Manufacturing Systems Engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China. zhouxiang@xjtu.edu.cn
Abstract:
A modified bi-dimensional empirical mode decomposition (BEMD) method is proposed for sparsely decomposing a fringe pattern into two components, namely, a single intrinsic mode function (IMF) and a residue. The main idea of this method is a modified sifting process which employs morphological operations to detect ridges and troughs of the fringes, and uses weighted moving average algorithm to estimate envelopes of the IMF, replacing respective local extrema detection and envelope interpolation of conventional BEMDs. The background intensity of the fringe pattern is automatically removed by extracting the single IMF, thereby relieving the mode mixing problem of the BEMDs. A fast algorithm based on 2D convolution is also presented for reducing the calculation time to several seconds only. This approach is applied to process simulated and real fringe patterns, and the results obtained are compared with Fourier transform, discrete wavelet transform, and other EMD methods. The MATLAB code is downloadable at http://gr.xjtu.edu.cn/web/zhouxiang.

