Laser divided-aperture differential confocal sensing technology with improved axial resolution

Area of Science:

• Optical Engineering
• Microscopy Technology
• Sensing Systems

Background:

• Confocal microscopy axial response is affected by detector offset.
• Divided-aperture systems present unique challenges for precise measurement.

Purpose of the Study:

• To introduce a novel laser divided-aperture differential confocal sensing technology (LDDCST).
• To achieve high axial resolution and absolute zero measurement capabilities.
• To develop an LDDCST-based sensor with enhanced performance.

Main Methods:

• Implementing two symmetrical micro-regions as virtual pinholes for spot-division detection.
• Utilizing differential subtraction of simultaneous intensity responses for signal processing.
• Adjusting virtual pinhole parameters in software to optimize resolution and measurement range.

Main Results:

• Demonstrated high axial resolution and absolute measurement capability.
• Achieved low noise levels through differential subtraction.
• Exhibited strong anti-interference and sectioning detection capabilities in experiments.

Conclusions:

• LDDCST provides a robust method for high-resolution axial measurements.
• The LDDCST sensor offers significant advantages in precision and noise reduction.
• This technology enhances the performance of confocal microscopy for advanced applications.